Journal: Emerging Microbes & Infections

Article Title: Specific determination of hepatitis B e antigen by antibodies targeting precore unique epitope facilitates clinical diagnosis and drug evaluation against hepatitis B virus infection

doi: 10.1080/22221751.2020.1862631

Figure Lengend Snippet: Kinetics of HBeAg production determined by various assays and the cccDNA association in drug-treated HepAD38 cells. HepAD38 cells were cultured and treated under the indicated conditions, culture medium were changed every 2 days and the supernatants at day 0 (D0), day 4 (D4), day 10 (D10) and day 14 (D14) since treatment administration were subjected to HBeAg measurements by the NTR-HBeAg assay (A), the Elecsys-HBeAg assay (B) and the Architect-HBeAg assay (C). At day 14 after treatment, the intracellular cccDNA levels were determined by qPCR (normalized by mtDNA quantification) and were shown in panel (D). Tet+, Tet presence; Tet-, Tet absence; ETV, Entecavir; Mor, Morphothiadin.

Article Snippet: Entecavir (ETV), Morphothiadin (Mor) and RG7834 were purchased from MedChemExpress.

Techniques: Cell Culture

Journal: iScience

Article Title: Whole-genome DNA methylation and DNA methylation-based biomarkers in lung squamous cell carcinoma.

doi: 10.1016/j.isci.2023.107013

Figure Lengend Snippet: Figure 6. The pyrosequencing results of 10 pairs of normal and LUSC tissues (A) Verification of four CpG sites in the diagnostic model. (cg14823851 from TBX4 matched P1 Pos. 1, cg02772121 from TRIM15 matched P2, cg10424681 from C6orf201 matched P3 Pos. 3, and cg12910906 from ARHGEF4 matched P4 Pos. 2). (B–D) The methylation levels of two CpGs after cg14823851 (B), four CpGs around cg10424681 (C), and two CpGs surrounding cg12910906 (D). Results are expressed as mean G SEM. N, normal (in blue); C, cancer (in red); P1, primer 1; Pos. 1, position 1. Data were presented as mean G SEM. Student’s t test was used. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Then antigen retrieval was conducted in citrate buffer using a microwave for 10 min. After cooling to room temperature, the sections were incubated with normal goat serum blocking solution for 20 min, and subsequently stained with anti-TBX4 antibody (#sc-515196; Santa Cruz Biotechnology, Shanghai, China) at a dilution of 1/10, anti-TRIM15 antibody (#13623-1-AP; ProteinTech, Wuhan, China) at a dilution of 1/200, anti-C6orf201 antibody (#sc-514971; Santa Cruz Biotechnology, Shanghai, China) at a dilution of 1/500, and anti-ARHGEF4 antibody (#55213-1-AP; ProteinTech, Wuhan, China) at a dilution of 1/50.

Techniques: Diagnostic Assay, Methylation

Journal: iScience

Article Title: Whole-genome DNA methylation and DNA methylation-based biomarkers in lung squamous cell carcinoma.

doi: 10.1016/j.isci.2023.107013

Figure Lengend Snippet: Figure 7. The protein levels of TBX4, TRIM15, C6orf201, and ARHGEF4 in LUSC (A–D) Representative images of human normal adjacent lung tissues and LUSC tissues stained with TBX4 (A), TRIM15 (B), C6orf201 (C), or ARHGEF4 (D) antibodies as indicated. Scale bar, 20 mm. (E) Immunohistochemical (IHC) staining of 4 antibodies in 10 pairs of normal and LUSC tissues was quantified by mean IOD. N, normal; C, cancer. Scale bar, 20 mm. Data were presented as mean G SEM. Paired Student’s t test was used. ***, p < 0.001.

Article Snippet: Then antigen retrieval was conducted in citrate buffer using a microwave for 10 min. After cooling to room temperature, the sections were incubated with normal goat serum blocking solution for 20 min, and subsequently stained with anti-TBX4 antibody (#sc-515196; Santa Cruz Biotechnology, Shanghai, China) at a dilution of 1/10, anti-TRIM15 antibody (#13623-1-AP; ProteinTech, Wuhan, China) at a dilution of 1/200, anti-C6orf201 antibody (#sc-514971; Santa Cruz Biotechnology, Shanghai, China) at a dilution of 1/500, and anti-ARHGEF4 antibody (#55213-1-AP; ProteinTech, Wuhan, China) at a dilution of 1/50.

Techniques: Staining, Immunohistochemical staining, Immunohistochemistry